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KCLB No. 01196
Name SNU-1196
Distributibility Yes
Cell Line STR
Profile
D3S1358: 14,15
vWA: 14,16
FGA: 24,25
Amelogenin: X
TH01: 5,7
TPOX: 10,11
CSF1P0: 10
D5S818: 11
D13S317: 9
D7S820: 8,11
Origin biliary tract
Species human
Cellular morphology epithelial
Growth pattern monolayer
Histopathology biliary tract cancer, hepatic duct bifurcation
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
References Ku et al., Establishment and characterization of six human biliary tract cancer cell lines, Brit J Cancer, 2002, 87:187-193
p53 mutation Arg273Cys
Kras codon 12 wild type
Populaton doubling time 48 hours
Depositor Jae-Gahb Park, M.D.
Originator Jae-Gahb Park, M.D.
Hit 984
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Cell viability 94%
Photos:Culture
Morphology, STR
Profiles