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KCLB No. 01079
Name SNU-1079
Distributibility Yes
Cell Line STR
Profile
D3S1358: 15
vWA: 16,18
FGA: 22
Amelogenin: X,Y
TH01: 8
TPOX: 8
CSF1P0: 12
D5S818: 10,13
D13S317: 8
D7S820: 8,12
Origin biliary tract
Species human
Cellular morphology epithelial
Growth pattern monolayer
Histopathology biliary tract cancer, intrahepatic duct
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
References Ku et al., Establishment and characterization of six human biliary tract cancer cell lines, Brit J Cancer, 2002, 87:187-193
Populaton doubling time 72 hours
Depositor Jae-Gahb Park, M.D.
Originator Jae-Gahb Park, M.D.
Hit 1027
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Cell viability 89%
Photos:Culture
Morphology, STR
Profiles