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KCLB No. 00308
Name SNU-308
Distributibility Yes
Cell Line STR
Profile
D3S1358: 16
vWA: 16
FGA: 25,26
Amelogenin: X
TH01: 8
TPOX: 8
CSF1P0: 12
D5S818: 9
D13S317: 8
D7S820: 7,12
Origin biliary tract
Species human
Cellular morphology epithelial
Growth pattern monolayer
Histopathology biliary tract cancer, gall bladder
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37'C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
References Ku et al., Establishment and characterization of six human biliary tract cancer cell lines, Brit J Cancer, 2002, 87:187-193
Kras codon 12 wild type
Populaton doubling time 48 hours
Depositor Jae-Gahb Park, M.D
Originator Jae-Gahb Park, M.D
Hit 910
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Cell viability 88 %
Photos:Culture
Morphology, STR
Profiles