[ List ]
Items Contents
KCLB No. 9S1937
Name HCC-1937
Distributibility Yes
Cell Line STR
Profile
D3S1358: 18
vWA: 16,17
FGA: 20,22
Amelogenin: X
TH01: 6,8
TPOX: 11
CSF1P0: 12
D5S818: 12
D13S317: 13
D7S820: 9,10
Origin breast
Species human - female, 24 years old, white
Cellular morphology epithelial
Growth pattern monolayer
Histopathology ductal cancer
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
References Gazdar et al., Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer, Int J Cancer, 1998, 78:766-774
Depositor Adi F Gazdar
Originator Adi F Gazdar
Hit 1069
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Photos:Culture
Morphology, STR
Profiles
no pictures.