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KCLB No. 10188
Name LS 174T
Distributibility Yes
Cell Line STR
D3S1358: 15,17
vWA: 15,17
FGA: 21,22
Amelogenin: X
TH01: 6,7
TPOX: 8,9
CSF1P0: 10,14
D5S818: 11,15
D13S317: 10,11
D7S820: 11
Origin colon
Species human - female, 58 years old, Caucasian
Reverse transcritase negative
Tumorigenecity yes, in nude mouse
Isoenzyme ADA,1:G6PD,B;PGM1,1;PGM3,2;PGD,A;ES-D,1;PEP-D,1
Cellular morphology epithelial
Histocompatibility HLA A2,B13,B50; Blood type O
Production CEA 1944ng/10exp6 cells in 10 days; mucin
Growth pattern monolayer
Histopathology adenocarcinoma; Dukes
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media MEM (Eagle) in Earle
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
References In Vitro 12:180-191, 1976
Note Colon Antigen 3 positive; derived from same tumor as ATCC CL 187
Hit 1410
Morphology, STR