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KCLB No. 10034
Name MDCK
Distributibility Yes
Cell Line STR
Profile
D3S1358:
vWA:
FGA:
Amelogenin:
TH01:
TPOX:
CSF1P0:
D5S818:
D13S317:
D7S820:
Origin kidney
Species canine - female, adult, Canis familiaris
Strain Cocker spaniel
Virus Susceptibility vesicular stomatitis (Indiana); vaccinia; coxsakievirus B5; reovirus 2.3; adenovirus 4,5; vesicular exanthema of swine; infectious canine hepatitis
Virus Resistance poliovirus 2; coxsackievirus B3, B4
Reverse transcritase negative
Cellular morphology epithelial
Production keratin
Growth pattern monolayer
Histopathology normal
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media MEM in Earle's BSS, 90%; heat inactivated fetal bovine serum (FBS), 10% or bovine calf serum (BCS), 10%
KCLB media Dulbecco's modified Eagle's medium, 90%; heat inactivated fetal bovine serum (FBS) 10%
Note The cells are positive for keratin by immunoperoxidase staining
Hit 3289
Photos:Culture
Morphology, STR
Profiles