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KCLB No. 10213
Name Daudi
Distributibility Yes
Cell Line STR
D3S1358: 16,18
vWA: 15,17
FGA: 21,26
Amelogenin: X,Y
TH01: 6,7
TPOX: 8,11
CSF1P0: 12
D5S818: 8,13
D13S317: 11,12
D7S820: 8,10
Origin lymphoblast
Species human - male, 16 years old, Black
Receptor complement; Fc of Ig G
Reverse transcritase negative
Tumorigenecity yes, , in nude mice; form colonies in agarose
Isoenzyme G6PD, B
Karyology normal male; diploid; stable
Cellular morphology lymphoblast
Growth pattern suspension
Histopathology lymphoma
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with 2mM L-glutamine adjusted to contain 1.5g/L sodium bicarbonate, 4.5g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80 %; heat inactivated fetal bovine serum (FBS), 20 %
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Cultures can be maintained by addition or replacement of fresh medium. Maintain cultures at 6 X 10 exp5 viable cells/ml.
References Gao Y et al . Introduction of an exceptionally high-level, nontranslated, Epstein-Barr virus-encoded polyadenylated transcript in the uurktts lymphoma line Daudi. J. Virol. 71: 84-94, 1997. Cuthbert JA and Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997
Note Surface immunoglobulin positive. The cells are negative for beta -2- microglobulin and are EBNA positive and VCA. The line carries Epstein-Barr virus
Hit 991
Morphology, STR
no pictures.