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KCLB No. 00410
Name SNU-410
Distributibility Yes
Cell Line STR
Profile
D3S1358: 15
vWA: 17
FGA: 23
Amelogenin: X
TH01: 9
TPOX: 8,12
CSF1P0: 12
D5S818: 12
D13S317: 10,12
D7S820: 12,14
Origin pancreas
Species human - male, 53 years old, Mongoloid
Cellular morphology epithelial
Growth pattern monolayer
Histopathology pancreatic carcinoma
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37'C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
References Ku et al., Establishment and characterization of 4 human pancreatic carcinoma cell lines: Genetic alterations in the TGFBR2 gene but not in the MADH4 gene, Cell Tiss Res,2002, 204-214
Kras codon 12 GGT->GTT
Populaton doubling time 72 hours
Depositor Jae-Gahb Park, M.D.
Originator Jae-Gahb Park, M.D.
Hit 1156
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Photos:Culture
Morphology, STR
Profiles