소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

SNU-1460

KCLB No. 01460
Name SNU-1460
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin colon; sigmoid colon
Species human, asian - male, 25 years old
Cellular morphology epithelial
Growth pattern monolayer
Histopathology adenocarcinoma
Differentiation moderately differentiated
Depositor Jae-Gahb Park, M.D.
Option culture only
D3S1358: 18
vWA: 14,18
FGA: 21,23
Amelogenin: X
TH01: 6,7
TPOX: 8,11
CSF1PO: 10,12
D5S818: 11
D13S317: 9,12
D7S820: 8,11
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Populaton doubling time 67 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Carcinogenesis, 31:1003-1009, 2010, Transl Oncol. 2019 Jun 19;12(9):1185-1195.

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