KCLB No. | 30037.1 |
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Name | Caco-2 |
Origin | colon |
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Species | human - male, 72 years old, caucasian |
Cellular morphology | epithelial |
Growth pattern | monolayer |
Histopathology | adenocarcinoma |
Option | culture only |
Freezing media | RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5% |
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Original media | Minimum essential medium (Eagle) in Earle |
KCLB media | Minimum essential medium, 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10% |
Subculture | Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37 |
Media change | Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days |
Korean Cell Line Bank, Korean Cell Line Research Foundation,
101, Daehak-ro, Jongno-gu, Seoul, 03080, Korea
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통신판매업 신고 제2015-서울혜화-0125호 | Tel : 82-2-3668-7915
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