Caco-2

KCLB No. 30037.1
Name Caco-2
세포주 분양조건
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수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin colon
Species human - male, 72 years old, caucasian
Cellular morphology epithelial
Growth pattern monolayer
Histopathology adenocarcinoma
Option culture only
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media Minimum essential medium (Eagle) in Earle
KCLB media Minimum essential medium, 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days

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