소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

SNU-2535

KCLB No. 02535
Name SNU-2535
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin Lung
Species human, asian - female, 57 years old
Growth pattern monolayer
Histopathology Non-small cell carcinoma, NOS
Depositor TM Kim
Option culture only
D3S1358: 15
vWA: 16,17
FGA: 24,27
Amelogenin: X
TH01: 7,9.3
TPOX: 11
CSF1PO: 11
D5S818: 10,12
D13S317: 9,11
D7S820: 11
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
Note ALK mutation, G1269A EML4(exon13)-ALK(exon20) fusion, FGFR4 (G388R)
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
J Thorac Oncol. 2013 Apr;8(4):415-422, Sci Rep. 2022 Apr 15;12(1):6345

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