SNU-228

KCLB No. 00228
Name SNU-228
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin kidney
Species human, asian - male, 51 years old, Mongoloid
Cellular morphology epithelial
Growth pattern monolayer
Histopathology renal cell carcinoma
Differentiation clear cell tye
Depositor Jae-Gahb Park, M.D.
D3S1358: 16
vWA: 14
FGA: 23
Amelogenin: X,Y
TH01: 6,9
TPOX: 8
CSF1PO: 11,12
D5S818: 10,12
D13S317: 11,13
D7S820: 10,12
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Populaton doubling time 24 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Shin et al., Establishment and characterization of seven human renal cell carcinoma cell lines, BJU internaltional. 2000, 85:130-137
  • Shin KH, Ku JL, Kim WH, Lee SE, Lee C, Kim SW, Park JG.: Establishment and characterization of seven human renal cell carcinoma cell lines. BJU Int. 85: 1, 130-138. Jan
    ▶ Ref. KCLB Cell line No.'s - [ 00228, 00267, 00328, 00333, 00349, 00482, 01272 ]

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