Korean Cell Line Bank

SNU-C5

KCLB No.	0000C5
Name	SNU-C5

세포주 분양조건: 비영리 동결(Frozen); 비영리 배양(Culture); 영리 동결(Frozen); 영리 배양(Culture)

서비스: DNA extraction service; cDNA synthesis service; Pellet service

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Information
STR data
Culture Morphology
NGS & Drug Info.
Culture Method
References

Origin	colon; colorectal
Species	human, asian - female, 77 years old
Cellular morphology	epithelial
Growth pattern	monolayer
Histopathology	adenocarcinoma
Differentiation	poorly differentiated
Depositor	Jae-Gahb Park, M.D.
Update date	2014-09-11

D3S1358: 15

vWA: 15

FGA: 19,23

Amelogenin: X

TH01: 6,9

TPOX: 8

CSF1PO: 11,13

D5S818: 11,12

D13S317: 8

D7S820: 12,13

Freezing media	RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media	RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
KCLB media	RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
Subculture	Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Note	Penicillin-streptomycin were added to culture media (final concentration, 100 U/mL)
Populaton doubling time	67 hours
Split ratio	1:4
Media change	Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days

Cancer Res, 47: 6710-6718, 1987

Rand A, Glenn KS, Alvares CP, White MB, Thibodeau SM, Karnes WE Jr: p53 functional loss in a colon cancer cell line with two missense mutations (218leu and 248trp) on separate alleles. Cancer Lett 2: 98(2), 183-91. Jan 1996
▶ Ref. KCLB Cell line No.'s - [ 0000C1, 0000C4, 0000C5 ]
Chung JK, Lee YJ, Kim C, Choi SR, Kim M, Lee K, Jeong JM, Lee DS, Jang JJ, Lee MC: Mechanisms related to [18F]fluorodeoxyglucose uptake of human colon cancers transplanted in nude mice. J Nucl Med 4: 2, 339-46. Feb 1999
▶ Ref. KCLB Cell line No.'s - [ 0000C2A, 0000C4, 0000C5 ]

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배양세포 중앙은행

NGS & Drug Test Info.
NGS INFO

Drug Screening Service
Cell based
Organoid based

Korean Cell Line Bank, Korean Cell Line Research Foundation,
101, Daehak-ro, Jongno-gu, Seoul, 03080, Korea
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