소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

SNU-216

KCLB No. 00216
Name SNU-216
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin stomach; lymph node
Species human, asian - female, 46 years old
Cellular morphology epithelial
Growth pattern monolayer
Histopathology adenocarcinoma
Differentiation moderately differentiated
Depositor Jae-Gahb Park, M.D.
D3S1358: 17
vWA: 14,20
FGA: 21
Amelogenin: X
TH01: 7
TPOX: 9
CSF1PO: 12
D5S818: 11
D13S317: 9
D7S820: 8,10
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
Note CEA, 9498 ng/10^6 cells in cell lysate, 219.6 ng/10^6 cells in supernatant; CA19-9, 48.6 unit/10^6 cells in cell lysate, 4.3 unit/10^6 cells in supernatant; TPA, 485505 unit/10^6 cells in cell lysate, 20786 unit/10^6 cells in supernatant.
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Park et al., Establishment and characterization of human gastric carcinoma cell lines, Int J Cancer. 1997 Feb 7; 70(4):443-449
  • Shin KH, Yang YM, Park JG: Absence or decreased levels of the hMLH1 protein in human gastric carcinoma cell lines: implication of hMLH1 in alkylation tolerance. J Cancer Res Clin Oncol 124: 8, 421-6.
    ▶ Ref. KCLB Cell line No.'s - [ 00001, 00005, 00016, 00216, 00484, 00520, 00601, 00620, 00638, 00668, 00719 ]

Korean Cell Line Bank, Korean Cell Line Research Foundation,
101, Daehak-ro, Jongno-gu, Seoul, 03080, Korea
대표자 : 박재갑 | 개인정보보안책임자 : 신영경 | 사업자 등록번호 : 208-82-03887
통신판매업 신고 제2015-서울혜화-0125호 | Tel : 82-2-3668-7915
Fax : 82-2-742-0021 | Email : kclb@kclb.kr

KCLB (C) is a registered mark of Korean Cell Line Bank. All rights reserved. 1998~2015