NCI-H146

KCLB No. 30173
Name NCI-H146
세포주 분양조건
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수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin lung; pleural effusion
Species human - male, 59 years old, Caucasian
Tumorigenecity yes; forms transplantable tumors in nude mice which histologically resemble tumor cells from the original biopsy specimen
Isoenzyme G6PD, B; PGM1, 1-2; PGM3, 1-2; ES-D, 1; Me-2, 2; AK-1, 1; GLO-1, 1
Karyology This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. The X chromosomes were paired, and no Y chromosome was detected n QM stained preparations
Cellular morphology epithelial
Growth pattern aggregates in suspension
Histopathology small cell lung cancer (SCLC)
D3S1358: 11
vWA: 14,16
FGA: 23
Amelogenin: X
TH01: 6,9.3
TPOX: 8,11
CSF1PO: 11
D5S818: 12
D13S317: 11,12
D7S820: 9,10
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640, 90%; heat inactivated fetal bovine serum (FBS), 10%(if desired, the serum can be reduced to 5%)
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Note the NCI-H146 cell line was derived by A.F. Gazdar and associates in 1979 from the pleural fluid of a patient with small cell cancer of the lung; the bone marrow specimen was taken prior to therapy; the line expresses elevated levels of four biochemical markers (neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity; the cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein; the cells produce relatively high amounts of c-myc mRNA, but c-myc DNA sequences are not amplified.
Cancer Res, 45:1187-1197, 1985; Cancer Res, 45:2913-2923, 1985

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