소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

HCC-95

KCLB No. 70095
Name HCC-95
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin lung
Species human - male, 65 years old
Cellular morphology epithelial
Growth pattern monolayer
Histopathology squamous cell carcinoma
Depositor Gazdar AF
D3S1358: 17
vWA: 14,17
FGA: 22
Amelogenin: X
TH01: 8,9
TPOX: 8,11
CSF1PO: 12
D5S818: 10,11
D13S317: 11
D7S820: 10
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Wistuba II et al., Comparison of features of human lung cancer cell lines and their corresponding tumors, Clin Cancer Res, 1999, 5:991-1000

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