소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

SNU-520

KCLB No. 00520
Name SNU-520
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin stomach
Species human, asian - female, 60 years old
Cellular morphology floating aggregates
Growth pattern suspension
Histopathology adenocarcinoma, primary
Differentiation poorly differentiated
Depositor Jae-Gahb Park, M.D.
D3S1358: 14,15
vWA: 17,18
FGA: 21
Amelogenin: X
TH01: 8,9
TPOX: 7,8
CSF1PO: 10,11
D5S818: 10,13
D13S317: 8
D7S820: 9,11
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
Note CEA, 25 ng/10^6 cells in cell lysate, 6 ng/10^6 cells in supernatant; CA19-9, 26.5 unit/10^6 cells in cell lysate, 0.8 unit/10^6 cells in supernatant; TPA, 126290 unit/10^6 cells in cell lysate, 6011.8 unit/10^6 cells in supernatant.
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Park et al., Establishment and characterization of human gastric carcinoma cell lines, Int J Cancer. 1997 Feb 7; 70(4):443-449
  • Shin KH, Yang YM, Park JG: Absence or decreased levels of the hMLH1 protein in human gastric carcinoma cell lines: implication of hMLH1 in alkylation tolerance. J Cancer Res Clin Oncol 124: 8, 421-6.
    ▶ Ref. KCLB Cell line No.'s - [ 00001, 00005, 00016, 00216, 00484, 00520, 00601, 00620, 00638, 00668, 00719 ]

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