Korean Cell Line Bank

SNU-620

KCLB No.	00620
Name	SNU-620

세포주 분양조건: 비영리 동결(Frozen); 비영리 배양(Culture); 영리 동결(Frozen); 영리 배양(Culture)

서비스: DNA extraction service; cDNA synthesis service; Pellet service

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Information
STR data
Culture Morphology
NGS & Drug Info.
Culture Method
References

Origin	stomach; ascites
Species	human, asian - female, 59 years old
Cellular morphology	scattered floating
Growth pattern	suspension
Histopathology	adenocarcinoma, metastatic
Differentiation	poorly differentiated
Depositor	Jae-Gahb Park, M.D.

D3S1358: 17,18

vWA: 16,18

FGA: 24

Amelogenin: X

TH01: 6,7

TPOX: 8,11

CSF1PO: 10,11

D5S818: 11

D13S317: 12

D7S820: 13

Freezing media	RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media	RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media	RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture	Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
Note	CEA, 252.9 ng/10^6 cells in cell lysate, 377 ng/10^6 cells in supernatant; CA19-9, 11.6 unit/10^6 cells in cell lysate, 2.7 unit/10^6 cells in supernatant; TPA, 80275 unit/10^6 cells in cell lysate, 6095 unit/10^6 cells in supernatant.
Split ratio	1:4
Media change	Collect the cells and centrifuge, remove two thirds of medium, add fresh medium (depending on cell density) every 2 to 3 days

Park et al., Establishment and characterization of human gastric carcinoma cell lines, Int J Cancer. 1997 Feb 7; 70(4):443-449

Shin KH, Yang YM, Park JG: Absence or decreased levels of the hMLH1 protein in human gastric carcinoma cell lines: implication of hMLH1 in alkylation tolerance. J Cancer Res Clin Oncol 124: 8, 421-6.
▶ Ref. KCLB Cell line No.'s - [ 00001, 00005, 00016, 00216, 00484, 00520, 00601, 00620, 00638, 00668, 00719 ]
Kang HC, Kim IJ, Park JH, Shin Y, Ku JL, Jung MS, Yoo BC, Kim HK, Park JG.: Identification of genes with differential expression in acquired drug-resistant gastric cancer cells using high-density oligonucleotide microarrays. Clinical Cancer Research 10: , 272-284.
▶ Ref. KCLB Cell line No.'s - [ 00620-5FU, 00620-ADR ]

배양세포 클러스터
배양세포 중앙은행

NGS & Drug Test Info.
NGS INFO

Drug Screening Service
Cell based
Organoid based

Korean Cell Line Bank, Korean Cell Line Research Foundation,
101, Daehak-ro, Jongno-gu, Seoul, 03080, Korea
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