소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

SNU-C4

KCLB No. 0000C4
Name SNU-C4
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin colon; colorectal
Species human, asian
Cellular morphology epithelial
Growth pattern monolayer
Histopathology adenocarcinoma
Differentiation poorly differentiated
Depositor Jae-Gahb Park, M.D.
Update date 2014-09-11
D3S1358: 16,20
vWA: 14,15
FGA: 20,21
Amelogenin: X,Y
TH01: 7,8
TPOX: 11
CSF1PO: 10
D5S818: 8,9
D13S317: 8,9
D7S820: 8
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Note Penicillin-streptomycin were added to culture media (final concentration, 100 U/mL)
Populaton doubling time 27 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Cancer Res, 47: 6710-6718, 1987
  • Rand A, Glenn KS, Alvares CP, White MB, Thibodeau SM, Karnes WE Jr: p53 functional loss in a colon cancer cell line with two missense mutations (218leu and 248trp) on separate alleles. Cancer Lett 2: 98(2), 183-91. Jan 1996
    ▶ Ref. KCLB Cell line No.'s - [ 0000C1, 0000C4, 0000C5 ]
  • Chung JK, Lee YJ, Kim C, Choi SR, Kim M, Lee K, Jeong JM, Lee DS, Jang JJ, Lee MC: Mechanisms related to [18F]fluorodeoxyglucose uptake of human colon cancers transplanted in nude mice. J Nucl Med 4: 2, 339-46. Feb 1999
    ▶ Ref. KCLB Cell line No.'s - [ 0000C2A, 0000C4, 0000C5 ]
  • Grem JL, Voeller DM, Geoffroy F, Horak E, Johnston PG, Allegra CJ: Determinants of trimetrexate lethality in human colon cancer cells. Br J Cancer 70: 6, 1075-84. Dec 1994
    ▶ Ref. KCLB Cell line No.'s - [ 0000C4, 90630 ]
  • Yee LK, Allegra CJ, Trepel JB, Grem JL: Metabolism and RNA incorporation of cyclopentenyl cytosine in human colorectal cancer cells. Biochem Pharmacol 1: 43(7), 1587-99. Apr 1992
    ▶ Ref. KCLB Cell line No.'s - [ 0000C4, 10247, 90630 ]
  • Grem JL, Allegra CJ: Sequence-dependent interaction of 5-fluorouracil and arabinosyl-5-azacytosine or 1-beta-D-arabinofuranosylcytosine. Biochem Pharmacol 5: 42(2), 409-18. Jul 1991
    ▶ Ref. KCLB Cell line No.'s - [ 0000C4, 10247 ]

Korean Cell Line Bank, Korean Cell Line Research Foundation,
101, Daehak-ro, Jongno-gu, Seoul, 03080, Korea
대표자 : 박재갑 | 개인정보보안책임자 : 신영경 | 사업자 등록번호 : 208-82-03887
통신판매업 신고 제2015-서울혜화-0125호 | Tel : 82-2-3668-7915
Fax : 82-2-742-0021 | Email : kclb@kclb.kr

KCLB (C) is a registered mark of Korean Cell Line Bank. All rights reserved. 1998~2015