소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

SNU-1076

KCLB No. 01076
Name SNU-1076
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin larynx, subglottic
Species human, asian - male, 60 years old
Abnormal genes p16, exon 2, codon 106, CCC->CAC (Pro to His)
Cellular morphology epithelial
Growth pattern monolayer
Histopathology carcinoma, squamous cell
Differentiation well differentiated
Depositor Jae-Gahb Park, M.D.
D3S1358: 15
vWA: 15,16
FGA: 24,25
Amelogenin: X,Y
TH01: 6,7
TPOX: 8,11
CSF1PO: 11,12
D5S818: 9,12
D13S317: 8,10
D7S820: 11,12
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Populaton doubling time 36 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Ku et al., Establishment and characterization of human laryngeal squamous cell carcinoma cell lines, The Laryngoscope (In press)

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