KCLB No. | 00449 |
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Name | SNU-449 |
Origin | liver |
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Species | human, asian - male, 52 years old |
Virus | Hepatitis B virus (HBV) |
Cellular morphology | epithelial |
Growth pattern | monolayer |
Histopathology | carcinoma, hepatocellular; primary |
Depositor | Jae-Gahb Park, M.D. |
D3S1358: 15,16 |
vWA: 14,16 |
FGA: 22,24 |
Amelogenin: X,Y |
TH01: 6,9 |
TPOX: 11 |
CSF1PO: 11 |
D5S818: 10 |
D13S317: 9 |
D7S820: 8,13 |
Freezing media | RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5% |
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Original media | RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10% |
KCLB media | AR5 |
Subculture | Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37 |
Note | Hepatitis B virus integrated. |
Populaton doubling time | 36 hours |
Split ratio | 1:4 |
Media change | Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days |
Park et al., Characterization of cell lines established from human hepatocellular carcinoma. Int J Cancer, 62: 276-282, 1995; Kang et al., Mutation of p53 gene in hepatocellular carcinoma cell lines with HBX DNA. Int. J. Cancer, 67: 898-902, 1996 |
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