SNU-503

KCLB No. 00503
Name SNU-503
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin colon; colorectal
Species human, asian - male, 52 years old
Cellular morphology epithelial
Growth pattern mololayer, or clumping
Histopathology carcinoma
Depositor Jae-Gahb Park, M.D.
D3S1358: 15
vWA: 14,17
FGA: 22
TH01: 9
TPOX: 8,11
CSF1PO: 11
D5S818: 10
D13S317: 10
D7S820: 10
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Populaton doubling time 64 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Oh et al., Establishment and Characterization of 12 human colorectal carcinoma cell lines. Int J Cancer, 81:902-910, 1999

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