Korean Cell Line Bank

SNU-283

KCLB No.	00283
Name	SNU-283

세포주 분양조건: 비영리 동결(Frozen); 비영리 배양(Culture); 영리 동결(Frozen); 영리 배양(Culture)

서비스: DNA extraction service; cDNA synthesis service; Pellet service

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Information
STR data
Culture Morphology
NGS & Drug Info.
Culture Method
References

Origin	colon; colorectal
Species	human, asian - female, 62 years old
Cellular morphology	epithelial
Growth pattern	monolayer, clumping
Histopathology	carcinoma
Depositor	Jae-Gahb Park, M.D

D3S1358: 15,18

vWA: 14,19

FGA: 23,25

Amelogenin: X

TH01: 7,9

TPOX: 8,11

CSF1PO: 11,13

D5S818: 9

D13S317: 8,11

D7S820: 11

Freezing media	RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media	RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media	RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture	Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Populaton doubling time	42 hours
Split ratio	1:4
Media change	Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days

Oh et al., Establishment and Characterization of 12 human colorectal carcinoma cell lines. Int J Cancer, 81:902-910, 1999

배양세포 클러스터
배양세포 중앙은행

NGS & Drug Test Info.
NGS INFO

Drug Screening Service
Cell based
Organoid based

Korean Cell Line Bank, Korean Cell Line Research Foundation,
101, Daehak-ro, Jongno-gu, Seoul, 03080, Korea
대표자 : 박재갑 | 개인정보보안책임자 : 신영경 | 사업자 등록번호 : 208-82-03887
통신판매업 신고 제2015-서울혜화-0125호 | Tel : 82-2-3668-7915
Fax : 82-2-742-0021 | Email : kclb@kclb.kr