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SNU-8

KCLB No. 00008
Name SNU-8
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin ovary; ascites
Species human, asian - female, 55 years old
Cellular morphology epithelial
Growth pattern monolayer
Histopathology cystadenocarcinoma, seroul, papillary
Depositor Jae-Gahb Park, M.D.
D3S1358: 14,16
vWA: 17
FGA: 26
Amelogenin: X
TH01: 9,9.3
TPOX: 8,11
CSF1PO: 11,12
D5S818: 11,12
D13S317: 8,9
D7S820: 8,12
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Note Prior chemotherapy : CAP ( cyclophosphamide, adriamycin, cisplatin). CA125 antigen was elevated in cell lysate and supernatant.
Populaton doubling time 44 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Yuan et al., Establishment and characterization of human ovarian carcinoma cell lines, Gynecol Oncol. 1997 Sep; 66(3): 378-387
  • Yuan Y, Kim WH, Han HS, Lee JH, Park HS, Chung JK, Kang SB, Park JG: Establishment and characterization of human ovarian carcinoma cell lines. Gynecol Oncol 66: 3, 378-87. Sep 1997
    ▶ Ref. KCLB Cell line No.'s - [ 00008, 00119, 00251, 00563, 00840 ]

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