소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

SNU-1079

KCLB No. 01079
Name SNU-1079
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin biliary tract
Species human, asian
Cellular morphology epithelial
Growth pattern monolayer
Histopathology biliary tract cancer, intrahepatic duct
Depositor Jae-Gahb Park, M.D.
Cell viability 89%
D3S1358: 15
vWA: 16,18
FGA: 22
Amelogenin: X,Y
TH01: 8
TPOX: 8
CSF1PO: 12
D5S818: 10,13
D13S317: 8
D7S820: 8,12
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Populaton doubling time 72 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Ku et al., Establishment and characterization of six human biliary tract cancer cell lines, Brit J Cancer, 2002, 87:187-193
  • Ku JL, Yoon KA, Kim IJ, Kim WH, Jang JY, Suh KS, Kim SW, Park YH, Hwang JH, Yoon YB, Park JG.: Establishment and characterisation of six human biliary tract cancer cell lines. Brit J Cancer 87: ,, 187-193. , 2002
    ▶ Ref. KCLB Cell line No.'s - [ 00245, 00308, 00478, 00869, 01079, 01196 ]

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