소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

SNU-308

KCLB No. 00308
Name SNU-308
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin biliary tract
Species human, asian - female,73 years old
Cellular morphology epithelial
Growth pattern monolayer
Histopathology biliary tract cancer, gall bladder
Depositor Jae-Gahb Park, M.D
Cell viability 88 %
D3S1358: 16
vWA: 16
FGA: 25,26
Amelogenin: X
TH01: 8
TPOX: 8
CSF1PO: 12
D5S818: 9
D13S317: 8
D7S820: 7,12
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
Populaton doubling time 48 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Ku et al., Establishment and characterization of six human biliary tract cancer cell lines, Brit J Cancer, 2002, 87:187-193
  • Ku JL, Yoon KA, Kim IJ, Kim WH, Jang JY, Suh KS, Kim SW, Park YH, Hwang JH, Yoon YB, Park JG.: Establishment and characterisation of six human biliary tract cancer cell lines. Brit J Cancer 87: ,, 187-193. , 2002
    ▶ Ref. KCLB Cell line No.'s - [ 00245, 00308, 00478, 00869, 01079, 01196 ]

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