소개 소식 자료실 Drug Screening Service STR,QC&서비스 특허&기탁 NGS & Drug Test Info. 생명연구자원 기탁등록보존 배양세포 클러스터 오가노이드분양 세포주분양

SNU-213

KCLB No. 00213
Name SNU-213
세포주 분양조건
서비스
수량
  • Information
  • STR data
  • Culture Morphology
  • NGS & Drug Info.
  • Culture Method
  • References
Origin pancreas
Species human, asian - male, 65 years old
Cellular morphology epithelial
Growth pattern monolayer
Histopathology pancreatic cancer
Depositor Jae-Gahb Park, M.D.
D3S1358: 15,16
vWA: 14,17
FGA: 23
Amelogenin: X,Y
TH01: 6,9
TPOX: 8,9
CSF1PO: 9,11
D5S818: 11,12
D13S317: 11
D7S820: 9
Freezing media RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
Original media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
KCLB media RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
Subculture Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
Populaton doubling time 48 hours
Split ratio 1:4
Media change Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Ku et al., Establishment and characterization of 4 human pancreatic carcinoma cell lines: Genetic alterations in the TGFBR2 gene but not in the MADH4 gene, Cell Tiss Res,2002, 204-214
  • Ku JL, Yoon KA, Kim WH, Jang Y, Suh KS, Kim SW, Park YH, Park JG.: Establishment and characterization of four human pancreatic carcinoma cell lines. Genetic alterations in the TGFBR2 gene but not in the MADH4 gene. Cell Tissue Res 308: ,, 205-214. , 2002
    ▶ Ref. KCLB Cell line No.'s - [ 00213, 00324, 00410 ]

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