Quality Control of Cell Lines
Since July 1st 2009, article publications on AACR journals including Cancer Research requires researchers to have DNA
fingerprinting analysis information using methods such as STR (Short Tandem Repeat) markers or others.
fingerprinting analysis information using methods such as STR (Short Tandem Repeat) markers or others.
For this reason, we provide DNA fingerprinting services as it was demanded by many researchers.
The analysis takes minimum of four weeks and cost is 150,000won($150) per sample.
If you would like to request this service please click on the request button below and fill out the request form.
This service is the test used when the contamination between different cell lines are being suspected.
This test enables the verification of the cell line origins (human, mouse, rat, hamster).
Cost is 20,000won($20) for 1 sample (after January, 2014).
If you would like to request this service please click on the request button below and fill out the request form.
Account number: 100-025-506327(Shinhanbank) Korean Cell Line Research Foundation
Mycoplasma grows on the cell surfaces. It is considered as a major problem in cell culture because contaminated cell lines have no notable change in its morphology and are not detected by the inverted microscope.
Mycoplasma contamination could affect gene expression, immune responses (in case of immune cell lines), and even genetic abnormalities. Above all, if not treated, mycoplasma can transmit through and contaminate other cell lines being cultured together. According to some reports, approximately 15 to 30 percent of cell lines that are being cultured in laboratories are mycoplasma contaminated.
If you would like to test your cell lines for the mycoplasma contamination, please print and fill out the cell line information form attached, and send it to KCLB with the DNA. Cost for the test is 10,000 per sample and DNA can be prepared in your common method.
DNA concentration should be 200 ng/ul or more, dissolved in water or rehydration buffer with minimum volume of 10ul and mailed to us via parcel delivery.
It takes maximum of 1 month for the result depending on the date of your request. After your deposit is checked result will be uploaded in pdf file on ‘request inquiry’ page. Information of your request would be protected.
If you would like to request this service please click on the request button below and fill out the request form.
Account number: 100-025-506327(Shinhanbank) Korean Cell Line Research Foundation Cost for the test 10,000won ($10) per sample [After January 2014]
Nucleic Acids & Cell pellets
This service allows researchers to acquire cell line DNA directly saving steps of cell culture and DNA extraction.
Sales unit is in 10ug (100ng, 100ul) for 300,000won ($300).
Please refer to 'DNA Panel of Cancer Cell Line' page to request this service.
If you would like to request this service please click on the request button below and fill out the request form.
Account number: 100-025-506327(Shinhanbank) Korean Cell Line Research Foundation
This service allows researchers to acquire cell line cDNA directly without having to go through the complex steps of cell culture, RNA extraction and cDNA synthesis. Researchers can now directly experiment, for example, RT-PCR for gene expression levels and other experiments, with synthesized cDNA.
Sales unit is in 10ug (100ng, 100ul) per cell line for 300,000won ($300). per cell line Please refer to ‘DNA Panel of Cancer Cell Line' section to request this service.
If you would like to request this service please click on the request button below and fill out the request form.
Established cell lines are being used not only in bioengineering area, but also in many other industries. Researches involving these cell lines require cell culture in order to acquire DNA, RNA and protein. During this process, many problems could occur such as technical, spatial, shortage in time, and contamination between cell lines, species, and microorganisms. To provide convenience on this matter, we culture the cell line and sell them out in pellet form. Minimum of 1x107 cells are inside a vial for 20,000won($200) per vial
If you want to request this service, please click request button below and fill out the request form.
Contract Research Organization (CRO)
Many cancer cell lines have abnormalities in di, tri, and tetra-nucleotide microsatellite resulting from the mutations of DNA repair genes or others. A typical case would be hereditary nonpolyposis colorectal cancer (HNPCC) where microsatellite instability (MSI) occur due to the mutation in DNA repair genes such as hMLH1 or hMSH2.
- KCLB tests MSI of the cell lines as it was demanded by many researchers.
- For MSI test, BAT-25, BAT-26 are being used as markers and 2ug of DNA is required.
- If you would like to request this service, please click on the request button below and fill out the request form.
- Cost for this test is 50,000won ($50). (After January 2014)
This service is for mass production of a cell line in Good Manufacturing Practice (GMP) facility. It is normally used to culture cell lines in absence of antibiotics or antimicrobials, to culture cells in sterilized environment, or to manufacture stocks for freezing or storing. You can request us to store cell lines in liquid nitrogen or in nitrogen vapor. This service proceeds in a unit of 100 vials. If you have any questions regarding this service please contact us by phone (02.3668-7915) or email (kclb@kclb.kr) Thank you.
KCLB can also provide other cell line related services that are not mentioned in service category. If you have any questions about our customized service, please call us (02.3668-7915) or send us an email. (kclb@kclb.kr) Thank you.
Hands-on Workshops
- 제1차세포주연구워크샵1998 (The 1st Korean Cell Line Research Workshop 1998), 2000월 02월 01일
- 제2차세포주연구워크샵1999 (The 2nd Korean Cell Line Research Workshop 1999), 2000월 02월 01일
- 제3차세포주연구워크샵2000 (The 3rd Korean Cell Line Research Workshop 2000), 2000월 02월 01일
- 제4차세포주연구워크샵2001 (The 4th Korean Cell Line Research Workshop 2001), 2001월 02월 01일
- 제5차세포주연구워크샵2002 (The 5th Korean Cell Line Research Workshop 2002), 2002월 02월 01일
- 제6차세포주연구워크샵2003 (The 6th Korean Cell Line Research Workshop 2003), 2003월 02월 01일
- 제7차세포주연구워크샵2004 (The 7th Korean Cell Line Research Workshop 2004), 2004월 02월 01일
- 제8차세포주연구워크샵2005 (The 8th Korean Cell Line Research Workshop 2005), 2005월 02월 01일
- 제9차세포주연구워크샵2006 (The 9th Korean Cell Line Research Workshop 2006), 2006월 02월 01일
- 제10차세포주연구워크샵2007 (The 10th Korean Cell Line Research Workshop 2007), 2007월 05월 16일
- 제11차세포주연구워크샵2008, 제14차유전성종양 및 유전체연구워크샵, 2008월 04월 10일
- 제12차세포주연구워크샵2009, 제15차유전성종양 및 유전체연구워크샵 2009, 2009월 04월 02일
- 13th Basic Cell Culture Techniques-Hands on Workshop, 2015월 01월 29일
- 14th Basic Cell Culture Techniques-Hands on Workshop, 2015월 07월 31일
- 15th KCLB Workshop Basic Cell Culture Techniques-Hands on Workshop, 2016월 01월 20일
- 16th KCLB Workshop, Basic Cell Culture Techniques Hands-on Workshop, 2016월 07월 18일
- 17차 KCLB Workshop, Basic Cell Culture Techniques Hands-on Workshop, 2017월 01월 11일
- 18th KCLB Workshop, Basic Cell Culture Techniques Hands-on Workshop, 2017월 10월 20일
- 19차 KCLB Workshop, Basic Cell Culture Techniques Hands-on Workshop, 2018월 01월 25일
- 20차 KCLB Workshop, Basic Cell Culture Techniques Hands-on Workshop, 2018월 07월 20일
- 21차 KCLB Workshop, Basic Cell Culture Techniques Hands-on Workshop, 2019월 01월 21일
- 22nd KCLB Workshop, Basic Cell Culture Techniques Hands-on Workshop, 2019월 07월 22일
- 23차 KCLB Workshop, Basic Cell Culture Techniques Hands-on Workshop, 2020월 01월 20일
- 제24차 KCLB Workshop 2022, The 12th Basic Cell Culture Techniques Hands-on Workshop, 2022월 07월 25일
- 제25차 KCLB Workshop 2022, The 12th Basic Cell Culture Techniques Hands-on Workshop, 2022월 08월 30일
- 제26차 KCLB Workshop 2022 -The 2nd Organoid Culture Techniques Hands-on Workshop, 2022월 10월 04일
- 제27차 KCLB Workshop 2023, The 13th Basic Cell Culture Techniques Hands-on Workshop, 2023월 01월 16일
- 제28차 KCLB Workshop 2023 -The 3rd Organoid Culture Techniques Hands-on Workshop, 2023월 02월 24일
- 제29차 KCLB Workshop 2023 -The 4th Organoid Culture Techniques Hands-on Workshop, 2023월 07월 17일
- 제30차 KCLB Workshop 2024-The 5th Organoid Culture Techniques Hands-on Workshop, 2024월 02월 26일
- 제31차 KCLB Workshop 2024-The 6th Organoid Culture Techniques Hands-on Workshop, 2024월 08월 28일
- 제32차 KCLB Workshop 2025 _The 7th Organoid Culture Techniques Hands-on Workshop, 2025월 02월 28일
Basic cell culture techniques
Primary cell culture
Quality control of cell lines
New training in organoid culture
Primary cell culture
Quality control of cell lines
New training in organoid culture
(To be completed by contributor of cell line.)
TO : KOREAN CELL LINE BANK
I understand that this material is for deposit in the Korean Cell Line Bank maintained at the Korean Cell Line Research Foundation.
It will be examined, and if accepted by th KCLB, batches will be made and distributed to the scientific community for a fee to cover expenses.
1. Depositor Information
| Depositor Name | |
|---|---|
| Deposited on behalf of | |
| Depositor Address | |
| Date | |
| Signature |
2. Cell Line Name
3. Source of cell (Species, Race, Sex, Age, Adult or Embryonal, Normal, Malignant, Organ or Tissue, Blood Type, etc)
4. Special Properties, Characteristics, or Uses
5. Type of Cell
6. Give reason why you feel this cell is important
7. Life Expectancy (Maximum number of serial subcultivation since origin)
8. Early passage levels available from frozen stock, If any
9. Name of originating or deriving investigator and date of origin
10. References
11. Brief history since origin
12. Recommended culture medium
13. Recommended subcultivation methods
14. Recommended for freezing cells, If any
