YD-8
| KCLB NO |
60501
|
|---|---|
| Name |
YD-8
|
세포주 분양조건
서비스
수량
-
+
| Origin |
oral, tongue
|
|---|---|
| Species |
human female,46 years old, Mongoloid
|
| Tumorigenecity |
no in athymic nude mouse, 0/5
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
squamous cell carcinoma
|
| Differentiation |
moderately differentiated
|
| Depositor |
Prof. Jong-In Yook
|
| DateOfUpdated |
2006-02-22
|
| D3S1358 |
OL,15.2
|
|---|---|
| vWA |
14,17
|
| FGA |
24
|
| Amelogenin |
X
|
| TH01 |
7,9
|
| TPOX |
8,11
|
| CSF1P0 |
10
|
| D5S818 |
12
|
| D13S317 |
8,10
|
| D7S820 |
10,13
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
The mixture of DMEM (3) : Ham's F12 (1) with 1x10^-10M cholera toxin, 0.4mg/ml hydrocortisone, 5ug/ml insulin, 5ug/ml transferrin, 2x10^-11M triiodothyronine(T3), 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| note |
The cancer cells were adapted to RPMI1640 medium supplemented with 10 % FBS. The original culture medium was replaced with KCLB medium.
|
| populaton doubling time |
29.3 hrs
|
| splitratio |
1:3
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Lee et al., Characterization of newly established oral cancer cell lies derived from six squamous cell carcinoma and two mucoepidermoid carcinoma cells. Exp Mol Med., 37(5) 379-390, 2005.
|
|---|
