NCI-H596
| KCLB NO |
90596
|
|---|---|
| Name |
NCI-H596
|
세포주 분양조건
서비스
수량
-
+
| Origin |
lung, soft tissue
|
|---|---|
| Species |
human male, 73 years old, Caucasian
|
| Abnormal genes |
p53 : LOH(+)
|
| Tumorigenecity |
yes, in nude mice
|
| Isoenzyme |
G6PD, B; PGM1, 1;PGM3, 1;ES-D, 1;Me-2, 0;AK-1, 1;GLO-1, 1;Phenotype Frequency Product = 0.0362
|
| Karyology |
This is a near triploid human cell line.
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
carcinoma, adenosquamous; stage 3A
|
| Modal Choromosome No |
71
|
| Depositor |
Adi F Gazdar
|
| DateOfUpdated |
1999-02-05
|
| D3S1358 |
14
|
|---|---|
| vWA |
16
|
| FGA |
23,25
|
| Amelogenin |
X
|
| TH01 |
7,9.3
|
| TPOX |
8
|
| CSF1P0 |
13
|
| D5S818 |
11,13
|
| D13S317 |
11
|
| D7S820 |
11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
RPMI1640, 90%; FBS 10%. Remove medium, add fresh 0.25% trypsin, 0.03% EDTA and let the culture sit at 37
|
| note |
This line was derived from a tumor mass in the chest wall of a patient with adenosquamous carcinoma of the lung. The specimen was obtained prior to therapy. The cells express easily detectable levels of p53 mRNA (comparable to levels found in normal lung), and exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin, but are negative for neurofilament triplet protein. The cells express multiple markers of squamous differentiation, including cross linked envelope formation, transglutaminase activity and production of involucrin.
|
| splitratio |
1:4 - 1:8
|
| media change |
2 to 3 times per week
|
|
Banks-Schlegel SP Et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. Takahashi T et al. P53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494. 1989. Levitt ML et al. Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Res. 50: 120-128, 1990.
|
|---|
