SW-900
| KCLB NO |
30059
|
|---|---|
| Name |
SW-900
|
세포주 분양조건
서비스
수량
-
+
| Origin |
lung
|
|---|---|
| Species |
human male, 53 years old, Caucasian
|
| Tumorigenecity |
Yes, produces tumors in nude mice consistent with type II bronchiolar adenocarcinoma
|
| Isoenzyme |
G6PD, B;PGM1,1;PGM3,2;ES-D,1;Me-2,2;AK-1,1;GLO-1,2
|
| Karyology |
hypotriploid
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
carcinoma, squamous cell; grade IV
|
| Modal Choromosome No |
56
|
| D3S1358 |
14
|
|---|---|
| vWA |
16
|
| FGA |
21,23
|
| Amelogenin |
X
|
| TH01 |
8
|
| TPOX |
11
|
| CSF1P0 |
11
|
| D5S818 |
11
|
| D13S317 |
8
|
| D7S820 |
11,12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Leibovitz's L-15 medium, 95%; heat inactivated fetal bovine serum (FBS), 5%
|
| kclb media |
Leibovitz's L-15, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or 37C) until the cells detach (about 10min).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
|
| splitratio |
1:2
|
| media change |
2 to 3 times per week
|
|
Fogh J et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J.Natl.Cancer Inst. 58: 209-214, 1977. Goodfellow M et al. One humdred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J.Natl.Cancer Inst. 59: 221-226, 1977
|
|---|
