MIA PaCa-2
| KCLB NO |
21420
|
|---|---|
| Name |
MIA PaCa-2
|
세포주 분양조건
서비스
수량
-
+
| Origin |
pancreas
|
|---|---|
| Species |
human male, 65 years old, Caucasian
|
| Isoenzyme |
G6PD, B
|
| Cellular morphology |
epithelial
|
| Production |
human colony stimulating factor, subclass I (CSF-1); plasminogen activator
|
| Growth Pattern |
monolayer with floating cells
|
| Histopathology |
carcinoma
|
| D3S1358 |
16
|
|---|---|
| vWA |
15
|
| FGA |
22
|
| Amelogenin |
X
|
| TH01 |
9,10
|
| TPOX |
9
|
| CSF1P0 |
10
|
| D5S818 |
12,13
|
| D13S317 |
12,13
|
| D7S820 |
12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
Dulbecco
|
| kclb media |
Dulbecco , FBS 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin for several minutes. Remove the trypsin and let the culture sit at 37
|
| note |
Hybridomas have been made against the CSF-1 produced by this line ATCC HB-8207(F1A3-23) and ATCC HB-8208 (F18 AF1).
The line is sensitive to asparaginase.
|
|
Wu MC et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. Fountzilas G et al . Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line(MIA PaCa-2). J. Nat
|
|---|
