세포주 정보

AsPC-1
AsPC-1
KCLB NO
21682
Name
AsPC-1
세포주 분양조건
서비스
수량
- +
Origin
pancreas; ascites
Species
human female, 62 years old, Caucasian
Production
carcinoembryonic antigen(CEA); human pancreas associated antigen; human pancreas specific antigen; mucin
Growth Pattern
monolayer
Histopathology
adenocarcinoma
D3S1358
16
vWA
17
FGA
24
Amelogenin
X
TH01
7,9.3
TPOX
8,10
CSF1P0
10,13
D5S818
12
D13S317
9,12
D7S820
12,13
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
RPMI1640 with sodium pyruvate, 80%; heat inactivated fetal bovine serum (FBS), 20%
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
subculturing
Remove medium, rinse monolayer with fresh 0.25 % trypsin, 0.02 % versene solution, remove trypsin and let the culture sit at 37
note
The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.
Chen WH et al . Human pancreatic adenocarcinoma: in vitro and in vivo morphology of a new tumor line established from ascites. In vitro 18:24-34, 1982