SNU-2335D
| KCLB NO |
02335D
|
|---|---|
| Name |
SNU-2335D
|
세포주 분양조건
서비스
수량
-
+
| Origin |
colon
|
|---|---|
| Species |
human, asian female, 55 years old
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer, polygonal
|
| Histopathology |
omental seeding nodule
|
| Option |
culture only
|
| D3S1358 |
16,18
|
|---|---|
| vWA |
14,17
|
| FGA |
26
|
| TH01 |
6,9
|
| TPOX |
8,11
|
| CSF1P0 |
11,12
|
| D5S818 |
10,13
|
| D13S317 |
11
|
| D7S820 |
12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
|
|
Establishment and Characterization of Paired Primary and Peritoneal Seeding Human Colorectal Cancer Cell Lines: Identification of Genes That Mediate Metastatic Potential., Transl Oncol. 2018 Oct;11(5):1232-1243.
|
|---|
