세포주 정보

SNU-2404A
SNU-2404A
KCLB NO
02404A
Name
SNU-2404A
세포주 분양조건
서비스
수량
- +
Origin
colon
Species
human, asian female, 62 years old
Growth Pattern
monolayer, Polygonal
Histopathology
Ascending colon mass
Option
culture only
D3S1358
17
vWA
14,18
FGA
23
Amelogenin
X
TH01
9
TPOX
8,9
CSF1P0
10,12
D5S818
11
D13S317
10,11
D7S820
10,12
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
subculturing
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
Establishment and Characterization of Paired Primary and Peritoneal Seeding Human Colorectal Cancer Cell Lines: Identification of Genes That Mediate Metastatic Potential., Transl Oncol. 2018 Oct;11(5):1232-1243.