HCC-1937BL
| KCLB NO |
9S1937BL
|
|---|---|
| Name |
HCC-1937BL
|
세포주 분양조건
서비스
수량
-
+
| Origin |
B cell; matched B lymphoblastoid cell line of HCC-1937
|
|---|---|
| Species |
human female, 24 years old, white
|
| Cellular morphology |
lymphoblastoid
|
| Growth Pattern |
suspension
|
| Histopathology |
Epstein-Barr virus transformed
|
| Depositor |
Adi F Gazdar
|
| EstablishedBy |
Adi F Gazdar
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Collect the cell in the 15ml tube, centrifuge, remove medium and add fresh medium
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Gazdar et al., Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer, Int J Cancer, 1998, 78:766-774
|
|
|---|---|
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