SNU-484
| KCLB NO |
00484
|
|---|---|
| Name |
SNU-484
|
세포주 분양조건
서비스
수량
-
+
| Origin |
stomach
|
|---|---|
| Species |
human, asian male, 53 years old
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
adenocarcinoma, primary
|
| Differentiation |
poorly differentiated
|
| Depositor |
Jae-Gahb Park, M.D.
|
| D3S1358 |
18
|
|---|---|
| vWA |
18
|
| FGA |
19,23
|
| TH01 |
7
|
| TPOX |
8
|
| CSF1P0 |
9
|
| D5S818 |
10
|
| D13S317 |
11,12
|
| D7S820 |
12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
|
| note |
CEA, 0.4 ng/10^6 cells in cell lysate, 0.1 ng/10^6 cells in supernatant; CA19-9, 9.9 unit/10^6 cells in cell lysate, 4.3 unit/10^6 cells in supernatant; TPA, 20.2 unit/10^6 cells in cell lysate, 9.1 unit/10^6 cells in supernatant.
|
| splitratio |
1:4
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Park et al., Establishment and characterization of human gastric carcinoma cell lines, Int J Cancer. 1997 Feb 7; 70(4):443-449
|
|
|---|---|
|
