세포주 정보

Ehrlich-Lettre Ascites, Strain E
Ehrlich-Lettre Ascites, Strain E
KCLB NO
10077
Name
Ehrlich-Lettre Ascites, Strain E
세포주 분양조건
서비스
수량
- +
Origin
Species
mouse
VirusSusceptibility
polyomavirus
Virus Resistance
poliovirus 3
Reverse Transcriptase
positive
Cellular morphology
epithelial
Growth Pattern
monolayer
Histopathology
carcinoma
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
NCTC 135 medium, 90%; heat inactivated fetal bovine serum (FBS), 10%
kclb media
Dulbecco
subculturing
Subcultures can be prepared by scraping, Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.Alternatively, a standard trypsinization procedure can be used.
note
Tested and found negative for ectromelia virus(mousepox)
Boone C et al. Characterization of an in vitro strain of Ehrlich-Lettre ascites carcinoma subjected to many periodic mouse passage. J. Natl. Cancer Inst. 34: 725, 1965.