SNU-2535
| KCLB NO |
02535
|
|---|---|
| Name |
SNU-2535
|
세포주 분양조건
서비스
수량
-
+
| Origin |
Lung
|
|---|---|
| Species |
human, asian female, 57 years old
|
| Growth Pattern |
monolayer
|
| Histopathology |
Non-small cell carcinoma, NOS
|
| Depositor |
TM Kim
|
| Option |
culture only
|
| D3S1358 |
15
|
|---|---|
| vWA |
16,17
|
| FGA |
24,27
|
| Amelogenin |
X
|
| TH01 |
7,9.3
|
| TPOX |
11
|
| CSF1P0 |
11
|
| D5S818 |
10,12
|
| D13S317 |
9,11
|
| D7S820 |
11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''''''''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
|
| note |
ALK mutation, G1269A
EML4(exon13)-ALK(exon20) fusion, FGFR4 (G388R)
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
J Thorac Oncol. 2013 Apr;8(4):415-422, Sci Rep. 2022 Apr 15;12(1):6345
|
|---|
