세포주 정보

SNU-2606
SNU-2606
KCLB NO
02606
Name
SNU-2606
세포주 분양조건
서비스
수량
- +
Origin
lung
Species
human, asian female, 51 years old
Cellular morphology
adherent
Growth Pattern
monolayer
Histopathology
adenocarcinoma
Depositor
Ja-Lok Ku
Option
culture only
D3S1358
15
vWA
17,19
FGA
20,26
Amelogenin
X
TH01
7,9
TPOX
8,11
CSF1P0
12,13
D5S818
9,13
D13S317
9
D7S820
9,12
준비중입니다.
freeze media
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
original medium
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
kclb media
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
subculturing
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37''C for 3 to 5 minutes, add culture medium and collect the cells, transfer the medium into 15ml tube, centrifuge, aspirate the medium, resuspend the pellets with culture medium and dispense into the culture flask
populaton doubling time
28 hours
media change
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
Culture and multiomic analysis of lung cancer patient‑derived pleural efusions revealed distinct druggable molecular types, Sci Rep. 2022 Apr 15;12(1):6345.