SNU-5
| KCLB NO |
00005.1
|
|---|---|
| Name |
SNU-5
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
stomach; ascites
|
|---|---|
| Species |
human, asian female, 33 years old
|
| Abnormal genes |
p53: codon 262-269, 24bp deletion, 8 amino acids
|
| Cellular morphology |
lymphoblast-like
|
| Growth Pattern |
suspension
|
| Histopathology |
adenocarcinoma
|
| Differentiation |
poorly differentiated
|
| Modal Choromosome No |
89
|
| Depositor |
Jae-Gahb Park, M.D.
|
| EstablishedBy |
Jae-Gahb Park, M.D.
|
| DateOfUpdated |
2014-09-11
|
| Option |
No Antibiotics
|
| D3S1358 |
16
|
|---|---|
| vWA |
15,16
|
| FGA |
21
|
| Amelogenin |
X
|
| TH01 |
9
|
| TPOX |
11
|
| CSF1P0 |
12
|
| D5S818 |
10
|
| D13S317 |
8
|
| D7S820 |
8,12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
|
| note |
No antibiotics were added to culture media (No penicillin and streptomycin)
|
| populaton doubling time |
34 hours
|
| splitratio |
1:4
|
| media change |
Collect the cells and centrifuge, remove two thirds of medium, add fresh medium (depending on cell density) every 2 to 3 days
|
|
Cancer Res, 50: 2773-2780, 1990; J Nat
|
|---|
