SNU-520
| KCLB NO |
00520.1
|
|---|---|
| Name |
SNU-520
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
stomach
|
|---|---|
| Species |
human, asian female, 60 years old
|
| Cellular morphology |
floating aggregates
|
| Growth Pattern |
suspension
|
| Histopathology |
adenocarcinoma, primary
|
| Differentiation |
poorly differentiated
|
| Depositor |
Jae-Gahb Park, M.D.
|
| Option |
No Antibiotics
|
| D3S1358 |
14,15
|
|---|---|
| vWA |
17,18
|
| FGA |
21
|
| Amelogenin |
X
|
| TH01 |
8,9
|
| TPOX |
7,8
|
| CSF1P0 |
10,11
|
| D5S818 |
10,13
|
| D13S317 |
8
|
| D7S820 |
9,11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
|
| note |
CEA, 25 ng/10^6 cells in cell lysate, 6 ng/10^6 cells in supernatant; CA19-9, 26.5 unit/10^6 cells in cell lysate, 0.8 unit/10^6 cells in supernatant; TPA, 126290 unit/10^6 cells in cell lysate, 6011.8 unit/10^6 cells in supernatant., No antibiotics were added to culture media (No penicillin and streptomycin)
|
| splitratio |
01:04
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Park et al., Establishment and characterization of human gastric carcinoma cell lines, Int J Cancer. 1997 Feb 7; 70(4):443-449
|
|---|
