SNU-479
| KCLB NO |
00479.1
|
|---|---|
| Name |
SNU-479
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
colon; sigmoid colon
|
|---|---|
| Species |
human, asian male, 62 years old
|
| Growth Pattern |
suspension
|
| Histopathology |
adenocarcinoma
|
| Differentiation |
moderately differentiated
|
| Depositor |
Jae-Gahb Park, M.D.
|
| Option |
No Antibiotics
|
| D3S1358 |
15
|
|---|---|
| vWA |
16,17
|
| FGA |
22,24
|
| TH01 |
6,7
|
| TPOX |
9,12
|
| CSF1P0 |
11,12
|
| D5S818 |
13
|
| D13S317 |
9,11
|
| D7S820 |
9,12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
|
| note |
No antibiotics were added to culture media (No penicillin and streptomycin)
|
| populaton doubling time |
44 hours
|
|
Carcinogenesis, 31:1003-1009, 2010
|
|---|
