SNU-C2A
| KCLB NO |
0000C2A.1
|
|---|---|
| Name |
SNU-C2A
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
colon; colorectal
|
|---|---|
| Species |
human, asian female, 43 years old
|
| Cellular morphology |
epithelial
|
| Growth Pattern |
monolayer
|
| Histopathology |
adenocarcinoma
|
| Differentiation |
poorly differentiated
|
| Depositor |
Jae-Gahb Park, M.D.
|
| DateOfUpdated |
2014-09-11
|
| Option |
No Antibiotics
|
| D3S1358 |
13
|
|---|---|
| vWA |
15,19
|
| FGA |
20,22
|
| Amelogenin |
X
|
| TH01 |
9
|
| TPOX |
8
|
| CSF1P0 |
11,14
|
| D5S818 |
8,9
|
| D13S317 |
9,12
|
| D7S820 |
13,16
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37
|
| note |
This cell line was derived from a 3rd passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy. This cell line is a pseudodiploid human cell line an and has a high concentration of receptors for EGF. This cell line has lost chromosomes 11 while SNU-C2B has lost only both chromosomes 8 and 11.
No antibiotics were added to culture media (No penicillin and streptomycin)
|
| populaton doubling time |
82 hours
|
| splitratio |
1:4
|
| media change |
Remove two thirds of medium and add fresh medium (depending on cell density) every 2 to 3 days
|
|
Cancer Res, 47: 6710-6718, 1987
|
|---|
