세포주 정보

13

15,18

20,23

X

9

8

11,14

8,9

9,10

13,16

RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%

RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%

RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%

Remove medium, add fresh 0.25 % trypsin 0.02 % EDTA solution, stand culture flask at 37

This cell line was derived from a 4th passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy. This cell line is a pseudodiploid human cell line an and has a high concentration of receptors for EGF. This cell line has lost both chromosomes 8 and 11 while SNU-C2A has lost only chromosome 11. No antibiotics were added to culture media (No penicillin and streptomycin)

1:4