COLO 320 HSR
| KCLB NO |
10220.1
|
|---|---|
| Name |
COLO 320 HSR
|
세포주 분양조건
서비스
수량
-
+
| Origin |
colon
|
|---|---|
| Species |
human female, 55 years old, Caucasian
|
| Reverse Transcriptase |
negative
|
| Tumorigenecity |
yes, in nude mice
|
| Isoenzyme |
PGM1, 1; PGM3, 2; G6PD, B; PEP-D, 1; 6PGD, A;ES-D, 1
|
| Karyology |
marker chromosomes with homogeneously staining regions were observed and the double minute chromosomes appeared in much lower frequency than the parental line COLO 320DM
|
| Cellular morphology |
rounded and refractile
|
| Production |
serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone(ACTH); parathyroid hormone
|
| Growth Pattern |
The cells grows loosely attached and in suspension
|
| Histopathology |
adenocarcinoma; Dukes
|
| D3S1358 |
17
|
|---|---|
| vWA |
15,18
|
| FGA |
20
|
| Amelogenin |
X
|
| TH01 |
8,9
|
| TPOX |
8,9
|
| CSF1P0 |
11
|
| D5S818 |
12
|
| D13S317 |
11
|
| D7S820 |
9,12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol. Subculture once per week.
|
| note |
CEA(-), CSAp(-), colon antigen 3(+); This line is a subline of COLO 320DM (KCLB 10220). The cells are weakly positive for keratins and vimentin
|
|
Clevenstine EC et al. Cell lines fromhuman colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924. 1979. Trainer DL et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988
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