SNU-769B
| KCLB NO |
00769B.1
|
|---|---|
| Name |
SNU-769B
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
colon; colorectal
|
|---|---|
| Species |
human male, 37 years old, Mongoloid
|
| Cellular morphology |
round, lymphoblastoid
|
| Growth Pattern |
suspension
|
| Histopathology |
carcinoma
|
| Depositor |
Jae-Gahb Park, M.D.
|
| Option |
No Antibiotics
|
| D3S1358 |
13,15
|
|---|---|
| TH01 |
7,8
|
| TPOX |
8
|
| CSF1P0 |
11,14
|
| D13S317 |
8,12
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
|
| note |
No antibiotics were added to culture media (No penicillin and streptomycin)
|
| populaton doubling time |
42 hours
|
| splitratio |
01:04
|
| media change |
Collect the cells and centrifuge, remove two thirds of medium, add fresh medium (depending on cell density) every 2 to 3 days
|
|
Oh et al., Establishment and Characterization of 12 human colorectal carcinoma cell lines. Int J Cancer, 81:902-910, 1999
|
|---|
