Molt-4
| KCLB NO |
21582
|
|---|---|
| Name |
Molt-4
|
세포주 분양조건
서비스
수량
-
+
| Origin |
T cell; T lymphoblast
|
|---|---|
| Species |
human male, 19 years old
|
| Cellular morphology |
lymphoblast
|
| Production |
high levels of terminal deoxynucleotidyl transferase are produced
|
| Growth Pattern |
suspension
|
| Histopathology |
leukemia, acute lymphoblastic
|
| D3S1358 |
14,16
|
|---|---|
| vWA |
17,18
|
| FGA |
22,23,24,25
|
| Amelogenin |
X,Y
|
| TH01 |
6,8
|
| TPOX |
8
|
| CSF1P0 |
11,12,13
|
| D5S818 |
12
|
| D13S317 |
12,13
|
| D7S820 |
8,10,11
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with 2mM L-glutamine adjusted to contain 1.5g/L sodium bicarbonate, 4.5g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Cultures can be maintained by additional or replacement of fresh medium.
Start new cultures at 4 X 10^5 cells/ml and subculture before the cell density reaches 2 X 10^6 cells/ml.
|
| note |
The cells do not produce immunoglobulin or Epstein-Barr virus.
There is a G->A mutation at codon 248 of the p53 gene.
P53 is not expressed. The line was originally contaminated with mycoplasma but has been cured with antibiotic.
The line was established from cells taken from a patient in relapse.
The patient had received prior multidrug chemotherapy.
MOLT-4 was derived from the same patient at the MOLT-3 cell line
|
|
Minowada J et al . Rosette-forming human lymphoid cell lines.1.Establishment and evidence for origin of thymus-derived lymphocytes. J. Nat'l. Cancer Inst. 49:891-895, 1972; Sandstrom PA and Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Nat'l . Acad. Sci. USA 90: 4708-4712, 1933.
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