SNU-487
| KCLB NO |
00487.1
|
|---|---|
| Name |
SNU-487
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
cervix, uterine
|
|---|---|
| Species |
human, asian female, 38 years old,
|
| Virus |
HPV type 18
|
| Cellular morphology |
floating aggregates
|
| Growth Pattern |
suspension
|
| Histopathology |
small cell carcinoma, stage IB (FIGO)
|
| Depositor |
Jae-Gahb Park, M.D.
|
| Option |
No Antibiotics
|
| Cell viability |
87%
|
| D3S1358 |
15,16
|
|---|---|
| vWA |
14,16
|
| FGA |
23
|
| Amelogenin |
X
|
| TH01 |
9,9.3
|
| TPOX |
11
|
| CSF1P0 |
11
|
| D5S818 |
10
|
| D13S317 |
8,12
|
| D7S820 |
10,13
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
|
| note |
HPV18, E1, deleted, E2, intact; HPV 18 was presumably integrated into chromosome; HPV 18 was not detectably by Southern blot analysis; E6/E7 gene of HPV 18 was expressed., No antibiotics were added to culture media (No penicillin and streptomycin)
|
| populaton doubling time |
52 hours
|
| splitratio |
01:02
|
| media change |
Collect the cells and centrifuge, remove two thirds of medium, add fresh medium (depending on cell density) every 2 to 3 days
|
|
Ku et al., Establishment and Characterization of 12 uterine cervical-carcinoma cell lines: Common sequence variation in the E7 gene of HPV-16-positive cell lines. Int J Cancer. 1997 Jul 17; 72(2):313-320
|
|---|
