SNU-1245
| KCLB NO |
01245.1
|
|---|---|
| Name |
SNU-1245
|
세포주 분양조건
- 무항생제 배양
서비스
수량
-
+
| Origin |
cervix, uterine
|
|---|---|
| Species |
human, asian female, 61 years old
|
| Virus |
HPV type 18
|
| Cellular morphology |
floating aggregates
|
| Growth Pattern |
suspension
|
| Histopathology |
small cell carcinoma, stage IIB (FIGO)
|
| Depositor |
Jae-Gahb Park, M.D.
|
| Option |
No Antibiotics
|
| Cell viability |
89%
|
준비중입니다.
| freeze media |
RPMI1640, 52.5%; FBS, 40%; DMSO, 7.5%
|
|---|---|
| original medium |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| kclb media |
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated fetal bovine serum (FBS), 10%
|
| subculturing |
Collect the cells into 15ml tube and centrifue, aspirate the culture medium, resuspend the pellets, dispense the cells into the culture flask
|
| note |
HPV18, E1 gene, intact, E2 gene, intact by PCR analysis; HPV 18 was presumably integrated into chromosome; HPV18 was not detectable by Southern blot analysis; E6/E7 gene of HPV18 was expressed by Northern analysis., No antibiotics were added to culture media (No penicillin and streptomycin)
|
| populaton doubling time |
54 hours
|
| splitratio |
01:02
|
| media change |
Collect the cells and centrifuge, remove two thirds of medium, add fresh medium (depending on cell density) every 2 to 3 days
|
|
Ku et al., Establishment and Characterization of 12 uterine cervical-carcinoma cell lines: Common sequence variation in the E7 gene of HPV-16-positive cell lines. Int J Cancer. 1997 Jul 17; 72(2):313-320
|
|---|
